Transcriptionally regulated growth-response genes play a pivotal role in the determination of the fate of a cell.p53 is known to transcriptionally regulate genes important in regulating cell growth potential. Using differ ential reverse transcription-PCR analysis of rat embryo fibroblast cells
نویسندگان
چکیده
Transcriptionally regulated growth-response genes play a pivotal role in the determination of the fate of a cell.p53 is known to transcriptionally regulate genes important in regulating cell growth potential. Using differ ential reverse transcription-PCR analysis of rat embryo fibroblast cells containing a temperature-sensitive p53 allele, we were able to isolate several transcripts up-regulated specifically in cells harboring functional PS3 protein. Two of these genes, SM2Oand microsomal epoxide hydrolase (mElt), are previously described genes. Two previously uncharacterized cDNAs, cell growth regulatory (CGR) genes CGRJJ and CGRJ9, were isolated. The predicted amino acid sequence of these novel proteins con tam knownmotifs;EF-handdomains(CGR11)and a ring-fingerdomaln (CGRJ9),suggestiveof function. CGRJJand CGRJ9appearto beprimary response genes expressed to moderate levels in functional p53 cells. Both CGRIJ and CGRJ9 are able to inhibit the growth of several cell lines.
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